Effect of mesenchymal stem cells and chicken embryo extract on flap viability and mast cells in rat skin flap

Farzaneh Chehelcheraghi,1,* Mohammadbayat,2 Sufan chien,3

1. Anatomical Sciences Department, School of Medicine, Lorestan University Medical of Sciences, Khoramabad, IR Iran,
2. Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3. Price Institute of Surgical Research, University of Louisville, and Noveratech LLC of Louisville, Louisville, Kentucky,



we investigated the effect of bone marrow mesenchymal stem cells (bmmscs) and chicken embryo extract (cee), alone and in combination, on tissue viability of skin flaps, and mast cells (mcs), in an experimental random skin flap (rsf) rat model


A30 mm×80 mm rsf was made on the dorsumofeachofthefortyrats,whichwerethendividedintofourgroups.onegroupdidnotreceiveanytreatment and served as the control, the second group received bmmscs, the third group received cee+bmmscs, and the fourth group received cee. for bmmsc treatment, 6×109 bmmscs were injected into twelve separate injection sites of each flap. seven days after rsf surgery, the remaining viable part of each flap was measured and examined to determine the number of blood vessels, mcs, and degranulated mcs.


:the cee, cee+ bmmsc,andbmmscgroupsdisplayedsignificantlyhigherlevelsofflapviability(anovatest:p=0.000;lsd testforallgroups:p=0.000),andagreaternumberofvessels(anovatest: p=0.000;lsdtest: p=0.000,0.002, and 0.012, respectively), compared with the control group. the flap viability was poorer in the bmmsc group thanintheceeandcee+bmmscgroups.thebmmscgroupalsohadagreaternumberofdegranulatedand total mcs, compared with the cee and cee+bmmsc groups.


we observed biostimulatory effects of bmmscs, cee, and cee + bmmscs on flap viability and vessel numbers, compared to the control group. mcs produced in response to bmmsc treatment have an inhibitory effect on the rsfs survival in an ischemic tissue model.


Ischemia-reperfusion injury; random skin flap; mast cell; bone marrow mesenchymal stem cell; chicken