Investigating the antimicrobial effects of nanoparticles bacteria isolated from agricultural territorys of ,investigating the antimicrobial effects of nanoparticles bacteria isolated from a

Mohammad Asadi,1,* Elnaz abedini,2

Abstract


Introduction

The use of microorganisms in the synthesis nanoparticles is known as an eco-friendly method. moreover, because of the ability of microorganisms to synthesize nanoparticles of various sizes, shapes and morphologies, this method has gained extreme attentions in recent years. the aim of this study was, therefore, to investigate the antimicrobial effects of nanoparticles synthesized by bacteria isolated from agricultural territorys of qazvin, iran.

Methods

This study was carried out in 2017. nanoparticles were characterized by sem, eds and xrd analyzes. the antimicrobial effects of nanoparticles were also assessed against some pathogenic bacteria.

Results

Of the 40 nanoparticle producing bacteria, the strains that were able to produce nanoparticles with high antimicrobial activity yielded under different environmental conditions, were selected. the results of scanning electron microscopy (sem) confirmed the presence of nanoparticles with a spherical shape. eds analysis showed that silver content of the particles was about 60 wt %. sequence alignment and phylogenetic tree results showed that m9 and b7 strains are closely related to bacillus cereus and pseudomonas argentinensis, respectively, with 99% homology.

Conclusion

The results showed that the m9 and b7 strains can synthesize nanoparticles with high antimicrobial effects under different environmental conditions.

Keywords

Biosynthesis, nanoparticles, bacillus cereus, antimicrobial effect