Investigating the effect of sclareol on ire-1 and perk genes in human mnk-45 gastric cancer cells

Elmira Aboutalebi vand beilankouhi,1 Ebrahim sahkinia,2,* Homayon dolatkhah,3 Mehran khojastehfard,4

4. 4. MSc in Clinical Biochemistry, Dept. of Clinical Biochemistry and Laboratories Medicine, Faculty of Medicine, Tabriz U



In recent years despite the decreasing incidence of stomach cancer. this disease is still the fourth leading cause of cancer death in the world. common cancer treatments may reduce the size of the tumor, but it is transient and does not have a positive effect on the survival of the patient and there is a risk of recurrence of the disease. strong induction of the endoplasmic reticulum stress has been shown to increase the susceptibility to anti-cancer treatment. considering the importance of medicinal plants in recent years, as well as the low side effects after administration, compared with synthetic drugs. the aim of this study investigated the effects of the cleaved sclareol from salvia officinalis on the induction of endoplasmic reticulum stress


The mkn-45 cell line was purchased from the pasteur institute of iran. this cells cultured in rpmi-1640 complete culture medium with cow serum. then cells cultured treated with 0, 20, 40, 60, 80 and 100 μm sclareol for 5 hours. the level of expression of ire-1 and perk genes was measured by quantitative real time-pcr


The expression level of ire-1 in doses of 20, 40 and 60 μm sclareol increased significantly compared with control group (p <0.0001). while decreasing in doses of 80 and 100 μm. also, the perk expression level in doses of 20, 40 and 60 μm sclareol showed a very high increase compared with control group (p <0.0001), but no increase was observed in doses of 80 and 100 μm.


The results of this study show that the doses of sclareol between 20 and 60 μm in increasing the amount of endoplasmic reticulum stress can be useful, but doses of sclareol above 60 μm do not.


Gastric cancer, sclareol, endoplasmic reticulum stress