1. Department of Biology, Tehran-east Payame Noor University, Tehran, Iran 2. Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, I. R. Iran
Gastric cancer is the 3rd common causes of cancer mortality among iranians. chronic infection with helicobacter pylori (h. pylori) is the most effective known risk factor for the development of gastric cancer.
among the mediators induced in response to the infection, micrornas (mirnas) have the potential role as a significant impact on the outcomes of the bacteria-host interaction. mirnas can play as either oncogenes or tumor suppressors. mirna expression could be modified by h. pylori infection; therefore, these can be used as biomarkers for gastric cancer. mir-375, down-regulated in h. pylori-infected gastric tumors, would be a possible biomarker of gastric cancer diagnosis.
Collection of validated and predicted target genes of mir-375 from mirtarbase and mirwalk databases, was filtered by unigene database to recognize their expression in gastric cancer tissue. gastric expressed targetome of mir-375 was selected for enrichment analysis in functional annotation tool, david.
David database including kegg signaling pathways showed target genes were significantly involved in cancer pathways further mapk signaling, tgf-beta signaling, wnt signaling and adherens junction pathways. comprehensive analysis of the coordinate expression of mirnas and mrnas reveals that mir-375 may play important role in the development of gastric cancer.
These signaling pathways lead to insensitivity to anti-growth signals, proliferation, angiogenesis and also evading apoptosis by irregulation in mtor signaling and hif-1 signaling pathway. however, limited studies on the role of h. pylori eradication in the impressed gene expression levels in gastric mucosa, such studies may reveal mirnas as molecular markers involved in inflammatory processes and gastric malignancy progression.
Gastric cancer, h. pylori, mir-375, cancer signaling pathway.