Comparative proteomes study of lactobacillus spp. for the identification of sortase-dependent proteins

Nahid Javanshir,1,* Najaf allahyari fard,2 Gholamreza ahmadian,3

1. National Institute of Genetic Engineering and Biotechnology(NIGEB)
2. National Institute of Genetic Engineering and Biotechnology(NIGEB)
3. National Institute of Genetic Engineering and Biotechnology(NIGEB)



In recent years, the extensive studies have been carried out to prove the therapeutic potential and beneficial of probiotics such as bifidobacteria and lactobacillus in human health. these human-friendly bacteria are important for healthy digestive tract and to balance of intestinal microbiome to avoid dysfunction of the human gut microbiome.


In this comparative study, genomics and proteomics evalutions of 118 lactobacillus spp. as probiotic and non probiotic lactobacillus were studied and the mechanisms of bacterial-intestinal cell wall interactions were investigated by focusing on diversity of sortase-dependent proteins (sdps).


These proteins are covalently coupling to the gram-positive bacterial peptidoglycan by sortase a enzyme through the conserved lpxtg motifs. genomes and proteomes wide screening based on these motifs showed that sdps are widely distributed in the either probiotic or non probiotic lactobacillus species and strains. the adhesive capacity of different probiotic strains to mucus and the gastrointestinal tract (git) may function to allow interactions with the local immune system in the(git) . recent interest in sortase and sdps has begun to focus on the role of these proteins in the immune modulatory. probiotics have the capacity to influence immune signaling of the colonic epithelium directly and through modulation of nf-κb signaling pathways. according to previous investigations some of probiotics such as l. rhamnosus gg and l. plantarum bfe have been shown to enhance innate immune signals by increasing the expression of toll-like receptors (tlrs) in ht-29 cells.


The hypothesis that sortase enzymes could play crucial roles in microorganism physiology further as mediating bacterial-host interactions has accelerated the study of this enzyme and its targets in different species of lactic acid bacteria(lab) . further investigation is required to find the specific sdps that are necessary for bacterial-host interactions in probiotic lactobacillus


Proteomes , lactobacillus , sortase-dependent proteins , sdps