Biosynthesis of silver nanoparticles by arum giganteum extract and antimicrobial effect of them
,1,* Seyed mohammad mehdi hamdi
,2 Behin omidi
1. Department of Biology,faculty of sciences, Central Tehran Branch, Islamic Azad University,Tehran, Iran
2. Department of Biology,faculty of sciences,Central Tehran Branch, Islamic Azad University,Tehran, Iran
3. Department of Biology,faculty of sciences,Central Tehran Branch, Islamic Azad University,Tehran, Iran
Now a day antimicrobial resistance is the major problem in the world, researchers try to replace new drugs. metal nanoparticles are is the one of the best alternative. nanoparticles, which refer to particles with the dimensions between 1 and 100 nm. among the nanoparticles, silver have been suitable chemical and physical characteristics and numerous applications in medicine and pharmacology. several methods used to synthesized silver nanoparticles(agnps), but each of these methods had disadvantages, chemical methods have high toxicity and low stability, and physical methods are costly and low-efficient. recently, researchers have found a new method for synthesized agnps, which used plants and microorganisms this method is called eco-friendly or green synthesis or biosynthesis. in this study we used arum giganteum extract to biosynthesis of agnps and antimicrobial effect of agnps biosynthesized were studied.
Extract of arum giganteum were exploited. then 4cc of this extract was added to 6cc silver nitrate 0.001m, ph 5.6 and incubated at 28 -35-370c. the formation of agnps was monitored by change color uv-vis spectroscopy, sem, xrd, ftir, dls. aa assay antimicrobial effect of agnps was studied on staphylococcus aureus atcc 1337, e.coli atcc 1398, klebsiella pneumonia atcc 1290, pseudomonas aeruginosa atcc 1181, bacillus subtilis atcc 6051 by disc diffusion method . for minimum inhibitory concentration test (mic) we used elisa method.
Result was shown that arum giganteum extract had good potentiality to biosynthesis of agnp. strong peak was observed at 420nm in uv-vis spectrophotometer, xrd and xrf results shows that agnps has been produced( strong pik in 111). size of this agnps was 38±1nm and their concentration was 620±0.5 ppm. antimicrobial test results include of staphylococcus aureus(15mm)(mic:250ppm), escherichia coli (16mm)(mic:500ppm), klebsiella pneumoniae(16mm)(mic:500ppm), pseudomonas aeruginosa(14mm)(mic:150 ppm ), bacillus subtilis(13mm)( mic : 500ppm) .
According to results, we have successfully extra cellular synthesized of agnps by arum giganteum extract. this way is simple, safe, low cost, effective and ecofriendly, the agnps synthesized using this method are very safe and can be used in pharmacology industry and medicine .
Silver nanoparticles, biosynthesis, arum giganteum