1. Malek Ashtar University of Technology, Tehran, Iran 2. Malek Ashtar University of Technology, Tehran, Iran 3. Tarbiat Modares University, Tehran, Iran 4. Malek Ashtar University of Technology, Tehran, Iran
Abstract
Introduction
Vibrio fischeri and vibrio harvey are the gram-negative bacteria of the vibrionaceae family that produce blue light and seen with the naked eye. the mechanism of light emission is dependence to an enzyme that named luciferase. these bioluminescence bacteria as biological markers could be used for measuring of food and water pollution. the aim of this work is the optimization of light emission of these bacteria using silver, silica and magnetic nanoparticles.
Methods
In this study, the effect of silver, silica and magnetic nanoparticles in the culture and light emission of two species examined v. fischeri and v. harvey was determined. for this, v. fischeri and v. harvey cultured in a seawater medium with different concentrations and type of nanoparticles at 25 ° c at 200 rpm. the growth rate and light emission of these bacteria was measured in 2 hour intervals using spectrophotometer and luminometer, respectively.
Results
The data was showed that silver and silica nanoparticles reduced the growth and decrease of light in two species. but, the effect of magnetic nanoparticle in two concentration of 50 and 150 µg/ml has been showed positive effects on growth rate and cause more stability of light from bioluminescence activity.
Conclusion
The culture optimization of these bacteria could be used for design of suitable kit in order to assessment of food and water pollution.
