1. Tehran university 2. Islamic Azad University of East Tehran 3. Islamic Azad University of East Tehran
Abstract
Introduction
Gastric cancer is one of the commonest malignant tumor worldwide and the highest mortality rates have been reported in east asia, including japan, korea, and china (28.1 per 100,000 males, 13.0 per 100,000 females). its treatment remains a challenge for physicians. the epidermal growth factor receptors are egfr, her2, her3 and her4. of the four epidermal growth factor receptors, egfr and her2 are well-known oncogenes involved in gastric cancer .egfr (her1) is a transmembrane protein that consists of an extracellular ligand binding domain, a transmembrane region and an intracellular domain with intrinsic tyrosine kinase activity. her1 and her2 overexpression are regarded as prognostic factors and targets of treatment.
Methods
In a case-control study, quantitative real-time pcr was used to analyze changes in her1 gene expression in formalin-fixed, paraffin-embedded of 25 t2d patients with gastric cancers and 25 controls. after slicing and deparaffinization, rna of samples was extracted and then cdna synthesis using of m-mulv enzyme and oligo dt and random hexamer primers were done. statistical analysis of research by graph pad prism software with the t-test procedure was done.
Results
results of real-time pcr showed her1 gene expression increased in most patient compared to normal control.(p value≤0.005) and by increasing the size of the tumor, the amount of gene expression in the patients increased significantly(p value≤0.005). it was determined that the expression of the her1 gene is directly related to progression in cancer stage..
Conclusion
Her1 overexpression is strongly associated with tumor progression and poor prognosis of patients with gastric cancer. egfr and her2 positivity are considered to be negative prognostic factors in gastric cancer. it may become a new prognostic factor and a target of treatment.
