1. Department of medical Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences 2. Department of Medical Biotechnology and Nanotechnology, School of Medicine, Zanjan University of Medical Sciences 3. Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences 4. Cellular and Molecular Biology Research Center, Shahide Beheshti of Medical Sciences,Tehran, Iran
Abstract
Introduction
Acinetobacter baumannii is a gram-negative nosocomial pathogen. multiple drug resistance (mdr) strain of a. baumannii makes treatment of infections very complicated. the development of vaccine is one of the most promising and cost-effective strategies to prevent infections. according to the studies, the ompa and bam antigens of a. baumannii, plays a major role in bacterial infectious processes. this study aimedto determine the potential immunogenic effect of poly-epitope constructs of ompa and bam antigens.
Methods
In the present study we designed and evaluated a 384 amino acid subunit of ompa and bam antigens by using bioinformatics site and tools. then cloned and expressed the synthetic constructs by using pet26b expression vector in e. coli bl21(de3). the protein was purified with ni-nta column. sds-page and western blot was used for confirmation of purified proteins. after purification of poly-epitopes, we evaluated humoral and cellular immune responses.
Results
Immunogenic and antigenic regions of ompa and bam proteins was found with proper score. sds-page and western blotting results indicated the similarity of in-silico designing and in vitro expression. humoral and cellular immune responses to poly-epitopes was determined by elisa.
Conclusion
We designed the novel poly-epitope constructs of ompa and bam antigens. the present study suggests that the poly-peptide based vaccine as potential candidates for protection against acinetobacter baumannii.
