1. Islamic Azad University, Hidaj Branch, Zanjan,Iran 2. Islamic Azad University, Zanjan Branch, Zanjan,Iran
Gastric cancer is one of the most common cancers that human societies face nowadays. aberrant expression of genes is one of important factor in cancer appearance. telomerase reverse transcriptase (htert in humans) is a catalytic subunit of the enzyme telomerase which, together with the telomerase rna, comprises the most important unit of the telomerase complex. telomerase lengthens telomeres in dna strands, thereby allowing senescent cells to continue cell division and become potentially immortal, as is often the case with cancerous cells. in this project we have studied htert expression in ags cell line which was treated with urtica extract. herbal extracts have been mentioned for fewer side effects in the treatment of diseases, including cancer.
Ags cells cultured in dmem with 10% fbs then treatment have done with concentrations of 800, 1200 and 2000 μg / ml of utrica extract for 48 hours. extraction of rna and cdna synthesis was performed using specific kits. the htert gene expression was measured by real time pcr.
The results showed the significant reduction of htert gene ( approx. 99% ) in all concentrations of urtica in ags cell line.
Htert may become therapeutic targets for cancer treatment and urtica as herbal extract decreased the expression rate of htert significantly.