مقالات پذیرفته شده در سومین کنگره بین المللی زیست پزشکی
Evaluation of in vitro detection methods of Mycoplasma Pneumoniae infections in recent years
Evaluation of in vitro detection methods of Mycoplasma Pneumoniae infections in recent years
Rasoul Kaviani,1Iman Pouladi,2,*
1. MSc in Medical Microbiology, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran. 2. MSc in Medical Microbiology, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
Introduction: Backgrounds: Mycoplasma pneumoniae is among the most common causes of community-acquired respiratory tract infections, accounting for 40% of pneumonia cases in children over 5 years of age. Diagnosis of M. pneumoniae infection is challenging due to fastidious growth requirements of the pathogen. The present study was carried out to provide a review of laboratory diagnostic methods of M. pneumoniae infection over the past few years.
Methods: Methods and materials: We collected the required data by reviewing papers indexed in Scopus, PubMed and Embase databases, as well as validated articles published in Iranian scientific journals over recent years. Key words such as Mycoplasma pneumoniae, diagnostic methods and review methods were used to collect the data.
Results: Results: Currently, various novel techniques have been used to detect M. pneumoniae infection, such as culture, serologic and molecular methods. Serology is an essential diagnostic tool most widely used to diagnose M. pneumoniae in clinical trials. Some of the serological methods used to detect M. pneumoniae constitute of cold hemagglutinin, complement fixation (CF) serologic diagnosis, and particle agglutination tests. Standard polymerase chain reaction (PCR) is currently a method of choice for direct detection of M. pneumoniae, and there are several PCR-based techniques that increase the sensitivity of accessibility and are successfully used for research purposes. These techniques include real-time nested PCR, reverse transcriptase PCR (RT-PCR) and multiplex PCR.
Conclusion: Conclusion: This paper reveals a summary of the current diagnostic status of M. pneumonia infection. Although, amplification-based methods have been replaced by hybridization and direct antigen detection methods, a mixture of both serological and direct pathogen detection methods must be applied for the detection of M. pneumonia infection.