مقالات پذیرفته شده در سومین کنگره بین المللی زیست پزشکی
Comparison of Loop-Mediated Isothermal Amplification and PCR to detect Yersinia pestis
Comparison of Loop-Mediated Isothermal Amplification and PCR to detect Yersinia pestis
Faezeh Houmansadr,1,*Mohammad Soleimani,2Keivan Majidzadeh,3
1. Department of Biology Cellular and Molecular, Science and Research branch, Islamic Azad University, Tehran, Iran 2. Department of Microbiology, Faculty of Medicine, AJA University of medical sciences, Tehran, Iran 3. Tasnim Biotechnology Research Center (TBRC), Faculty of Medicine, AJA University of medical sciences, Tehran, Iran
Introduction: Plague is a vector-borne disease caused by Yersinia pestis and transmitted by fleas from rodent reservoirs. Also, the possible illegitimate use of Y. pestis as a weapon, demands the urgent development of plague rapid detection. We show here comparison of Loop-Mediated Isothermal Amplification (LAMP) and PCR methods for detecting of the Capsular Antigen F1 (Caf1) gene of Y. pestis.
Methods: The Caf1-PCR and Caf1-LAMP assays were compared by their analytical specificity and sensitivity for detection of Caf1 gene Y. pestis bacteriae.
Results: The specificity of LAMP and PCR were 100%. The results have shown that the Caf1-LAMP was more sensitive than Caf1-PCR.
Conclusion: The LAMP described here, has the potential to become a standardized method for the rapid detection of Y. pestis with high sensitivity and specificity and cost-effective.