مقالات پذیرفته شده در سومین کنگره بین المللی زیست پزشکی
Oncogenic CCAT1 (Colon-cancer associated transcript-1) LncRNA in comparing with Tumor suppressor GAS5 (growth arrest-specific transcript 5) LncRNA in Breast cancer patients
Oncogenic CCAT1 (Colon-cancer associated transcript-1) LncRNA in comparing with Tumor suppressor GAS5 (growth arrest-specific transcript 5) LncRNA in Breast cancer patients
Fatemeh Akhavan Attar,1,*Maryam Yousefpour,2Zahra Pourramezan,3Masoumeh Azizi,4Mana Oloomi,5Shiva Irani,6
1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran 2. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran 3. Molecular Biology Department, Pasteur Institute of Iran, Tehran, Iran 4. Department of Molecular Medicine; Biotechnology Research Center; Pasteur Institute of Iran, Tehran, Iran 5. Molecular Biology Department, Pasteur Institute of Iran, Tehran, Iran 6. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Introduction: Breast cancer (BC) is a heterogeneous disease. It is the most cause of cancer-mortality among women all over the word. New biomarkers identification and methods are essential for BC treatment. Long non-coding RNAs (LncRNAs) are biomarkers that can effect in BC as tumor suppressor genes or oncogenes.
Methods: In this study, blood specimens were collected from 30 normal healthy women and 30 breast cancer patients. RNA was extracted from the whole blood and then cDNA was synthesized. Real-Time PCR technique was used for measuring the expression level of CCAT1 (Colon-cancer associated transcript-1) and GAS5 (growth arrest-specific transcript 5) LncRNAs.
Results: Expression level of CCAT1 LncRNA was significantly increased while GAS5 LncRNA expression was decreased in BC patients. Then, the level of expression was compared in patients and normal women
Conclusion: The key roles of LncRNAs in biological regulation have been recognized in many kinds of cancers. Up-regulated CCAT1 and down-regulated of GAS5 LncRNAs in Iranian BC patients can be used as BC biomarkers. LncRNA deregulations can also be used in BC treatment, in future.
Keywords: Breast cancer, LncRNA, whole blood, CCAT1, GAS5, Real-time PCR