Introduction: Phoenix dactyliferous L. commonly known as date palm that is used to improve fertility in men and women in Middle East folk medicine. These natural compounds consist of amino acid, fatty acid, oestrone, flavonoids and vitamins. According to the role of granulosa cells in female reproduction, aim of this study was to investigate the effect of date palm pollen grain extract on mice granulosa cells.
Methods: In this study granulosa cells isolated from female NMRI mice ovaries aging 21-25 days. Granulosa cells were cultured in α-MEM with different concentrations 20, 30, 40, 50, 100,200,300,500 μg/ml and 0.01 g/ml of palm pollen grain extract without media for 48 h. Then, the effect of date palm pollen grain on granulosa cell viability were analyzed with MTT assay.
Results: Our findings exhibited that there was increase in granulosa cells proliferation rate in all treated groups as compared with control group (p), but proliferation rate was significantly higher in the presence of 0.01 g/ml palm pollen grain.
Conclusion: Another studies demonstrated that DPP can improve various parameter in male and female reproductive system. Our data revealed that DPP can effect on ovarian folliculogenesis with increase proliferation rate in mice granulosa cells. In addition, it is interesting that DPP as feeder media can enhance cell quantity in the absence of α-MEM.
Keywords: Date palm pollen grain. infertility, granulosa cella, Folliculogenesis