مقالات پذیرفته شده در سومین کنگره بین المللی زیست پزشکی
Investigation the Dosage Dependency of Thyroxin (T4) on Squamous Cell Carcinoma
Investigation the Dosage Dependency of Thyroxin (T4) on Squamous Cell Carcinoma
Mohammad ali pahlevan neshan,1Amin Ebrahimi Sadrabadi,2Arsalan jalili,3,*
1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran 2. Cancer Biomedical Research Center, Tehran, Iran (CBC) 3. Hematopoietic Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Introduction: Preclinical evidence designates that the thyroid hormone can promote tumor cell proliferation, antiapoptosis, and cancer-associated angiogenesis. However, strong evidence exists that T4 can act at the plasma membrane to help via downstream signal transduction a considerable panel of intranuclear events, including specific gene transcription and activation of TR, but the exact mechanism of these actions of the hormone is still unknown.
Thyroxin (T4) is a kind of Thyroid hormone which regulates cell proliferation and cancer development. The functional dose of Thyroxin (T4) in several cancer is different so, the aim of this study is the investigation of thyroxin (T4) on cell viability of Squamous Cell Carcinoma cell lines (PC9) and analysis its dosage dependency.
Methods: In this study, PC9 cell lines were divided into nine groups, a control group, and eight groups, which treated via doses 0.1, 0.5, 1, 5, 10, 20, 30, 40 Lambda (λ) of T4. MTT assay has been done to analyze the cytotoxicity of thyroxin. Then the final result was analyzed by one-way ANOVA.
Results: The result demonstrated that 1 (λ) and 5 (λ) dosages of T4 illustrate the high cytotoxicity and low cytotoxicity of thyroxin were in 0.5 and 40 (λ) dosages.
Conclusion: The results of this study showed that the toxicity of thyroxin is not depended on thyroxin dosages on Squamous Cell Carcinoma cell lines (PC9), which suggesting thyroxin effect on lung cancer cell need more study.