مقالات پذیرفته شده در سومین کنگره بین المللی زیست پزشکی
Investigation of anti-herpetic capparis spinosa leaf extract effect in cell culture conditions
Investigation of anti-herpetic capparis spinosa leaf extract effect in cell culture conditions
marzieh taherzadeh,1,*
1. Bushehr University Of Medical Sciences, School of paramedical sciences, Bushehr, Iran
Introduction: HSV-1 causes a highly contagious infection, which is common and endemic throughout the world. The virus able to be implanted in nerve cells, and thus reactivates in the presence of environmental or internal stimuli. It is also one of the most important opportunistic infections in immunocompromised individuals. Due to the emergence of drug-resistant strains, the side effects of the main drug (acyclovir), the researchers have always been looking for an alternative and effective drug against the virus. In the present study, the effect of raw leaves extract of native C.spinosa in Bushehr province on the virus has been investigated.
Methods: Intact leaves of C. spinosa were dried at room temperature and extracted with three solvent (n-hexane, ethyl acetate and methanol) during overnight repeatedly shaking. Seven serials of the extract rootstock (0.1 g/ml) were prepared with 1:2 ratios and treated with Vero cells for their cytotoxicity effects. The anti-viral activity of the extracts were also evaluated based on MNTC (maximum non-toxic concentration).
Results: CC50 with ethyl acetate and methanol extracts were obtained in the third and second serials with 11.4 μg/ml and 22.6 μg/ml respectively. MNTC for the both extracts was obtained in the fifth series with 3.1 μg/ml concentration. IC50 for pre-attachment and post penetration stages of the virus pathogenesis was calculated 0.4 μg/ml and 0.64 μg/ml, 1 μg/ml and 0.74 μg/ml respectively. Furthermore Selectivity Index (SI) of these extracts was calculated 28.5 and 35.4 in the pre-attachment and 11.4 and 30.6 in the post penetration stages, Likewise.
Conclusion: According to the lower toxicity of methanol extract and obtained SI for ethyl acetate and methanol extract on the different stages of virus infectivity, it can be concluded that the methanol extract of C. spinosa is more effective than its ethyl acetate extract and is more likely to be considered for an alternative drug candidate and studied more precisely at the molecular level.