• Comparison of antibacterial effect of green and chemical synthesis of silver nanoparticles
  • Niloofar Hajarian Rezazadeh,1,* Foad Buazar,2 Soheila Matroodi,3
    2. a Department of Marine Chemistry, Khorramshahr University of Marine Science and Technology, Khorramshahr, PO. Box 669, Iran
    3. a Department of Marine Chemistry, Khorramshahr University of Marine Science and Technology, Khorramshahr, PO. Box 669, Iran


  • Introduction: Over the past few decades, metal nanoparticles less than 100 nm in diameter have made a substantial impact across diverse biomedical applications. silver nanoparticles have received much attention because of their antibacterial potential. In this study, the antibacterial properties of green synthesis nanoparticles and chemical synthesis were compared on pathogenic bacteria K.Pneumoniae, sp. Proteus, Salmonella sp. E. coli and Bacillus cereus.
  • Methods: AgNPs practically were biosynthesized by mixing 5% chitosan, and 5% silver nitrate in an autoclave at 120 °C and pressure of 15 psi for 15 minutes. The disk method was used to investigate the antibacterial property of the same Concentration nanoparticles on gram-negative bacteria K. pneumoniae, sp. Proteus, Salmonella sp. E. coli and gram-positive Bacillus cereus bacteria were added.
  • Results: In this synthesis, electron-rich organic compounds in the chitosan solution were acted as a reducing and stabilizing agent for silver nanoparticles. To reach the best conditions for synthesis, effective parameters in reaction were optimized. UV-Vis, FTIR, DLS, XRD, and TEM techniques were utilized for characterization the as-biosynthesized silver nanoparticles. Results clearly have shown that biological nanoparticles have relatively higher antimicrobial activity than that of chemical silver nanoparticles and And there was a significant difference diameter of the zone of inhibition between the two AgNPs.
  • Conclusion: This one-step method is facile and completely green without the use of harmful chemical substances. Hence, the produced AgNPs can be used as green antibacterial drugs instead of corresponding hazardous chemical ones.
  • Keywords: Green synthesis, Silver nanoparticles, Antimicrobial activity, chemical synthesis