• Antibacterial activity of silver nanoparticles against Salmonella enterica and Bacillus Cereus
  • Niloofar Hajarian Rezazadeh,1,* Foad Buazar,2 Soheila Matroodi,3
    2. a Department of Marine Chemistry, Khorramshahr University of Marine Science and Technology, Khorramshahr, PO. Box 669, Iran
    3. a Department of Marine Chemistry, Khorramshahr University of Marine Science and Technology, Khorramshahr, PO. Box 669, Iran


  • Introduction: Since nanoparticles in the life cycle and ecosystem have shown the least toxicity, they are a good choice for the elimination of pathogenic microbes, so silver nanoparticles(AgNPs) can be used as antibacterial agents. In this study, the green synthesis of AgNPs and the antibacterial effect of it on Bacillus Cereus and Salmonella enterica bacteria were investigated.
  • Methods: AgNPs practically were biosynthesized by mixing aqueous extracts of brown algae Sargassum, 5% chitosan, and 5% silver nitrate in an autoclave at 120 °C and pressure of 15 psi for 15 minutes. Antimicrobial effect AgNPs for Bacillus Cereus and Salmonella enterica are investigated by using the disk diffusion method.
  • Results: The first characteristic of nanoparticles production was the rapid change in color of the solution from yellowish to brown to dark brown. UV-Vis further confirmed the formation of AgNPs showing maximum absorption peak at 420 nm wavelength. FTIR spectrum indicated the regenerative functional groups engaged in silver ions bioreduction in a mixture of chitosan and algae extract. Transmission electron microscopy results showed that the nanoparticles produced were spherical shape with the particle size range of 20-30 nm. Green synthesized AgNPs show high and efficient antimicrobial activities against two human pathogens (Bacillus Cereus and Salmonella enterica) and And there was no significant difference diameter of the zone of inhibition between the two strains.
  • Conclusion: Easy to workout, readily available and compatible in the environment are the main advantages of the proposed green route and could be utilized as antimicrobial agents for effective disease management.
  • Keywords: Greensyntesis, Silver nanoparticles, Antimicrobial activity, Human pathogen