مقالات پذیرفته شده در سومین کنگره بین المللی زیست پزشکی
Evaluation Of down Syndrome, Pato and Edward Syndrome in fetal specimens using QF PCR
Evaluation Of down Syndrome, Pato and Edward Syndrome in fetal specimens using QF PCR
Asmaolhosna Amini,1,*Hasan Bahmani,2Tahmine porrajab,3Sara Talebi,4Saedeh Ravandeh,5
1. Human Genetic Research Center, Baqiyatallah University of Medical Science, Tehran, Iran 2. Human Genetic Research Center, Baqiyatallah University of Medical Science, Tehran, Iran 3. Human Genetic Research Center, Baqiyatallah University of Medical Science, Tehran, Iran 4. Human Genetic Research Center, Baqiyatallah University of Medical Science, Tehran, Iran 5. Human Genetic Research Center, Baqiyatallah University of Medical Science, Tehran, Iran
Introduction: The qf pcr method is a method of evaluating fluorescence on STR markers on chromosomes 21, 13, 18, and personality for chromosomal consulting in Down, Pato, Edward, which have subsequently been successfully promoted and elevated. Medical Genetics Laboratories Frequently Used.
Methods: In this study, this test was performed on 1000 embryonic samples using devyser kit, firstly on dna extracted samples, then amplified with 26 pairs of labeled primers in 4 fluorescent multiplex colors. Genetic Analyzer 3130xl was read and its results evaluated in gene mapper software.
Results: Of the 1000 samples examined, 20 were Down Syndrome (Trisomy 21), 3 were Pato Syndrome (Trisomy 13), 1 was Edvard Syndrome (Trisomy 18) and the rest were normal. Percentage of Edward's syndrome.
Conclusion: The qf pcr test is usually performed in conjunction with a cytogenetic test due to its high speed in detecting fetal aneuploidy problems until a specific karyotype test is performed to track abortion licenses if seen by a physician. It has high accuracy and speed and is reasonably priced, but the key to this test is the use of quality kits, experienced personnel and appropriate equipment.