مقالات پذیرفته شده در سومین کنگره بین المللی زیست پزشکی
Study of lncRNA TMPO-AS1 expression in gastric cancer
Study of lncRNA TMPO-AS1 expression in gastric cancer
elaheh mohammadali,1Reza Safaralizadeh,2,*behzad baradaran,3
1. Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz., Tabriz., Iran 2. Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz., Tabriz., Iran
Introduction: Gastric cancer is one of the most common cause of cancer-related death; besides, the diagnosis and therapy of it are still challenging. Researches have revealed that long non-coding RNAs(lncRNAs) play important roles in the genesis and progression of various human cancers including gastric cancer (GC). Investigations showed that TMPO-AS1 may be a diagnostic and prognostic marker in multiple cancer types. Thus, we selected TMPO-AS1 for further study in gastric cancer.
Methods: In the present study, solid tissue samples from 20 gastric tumors, and 20 normal tissues were obtained from gastric cancer patients. Afterward, the extracted RNAs were reverse-transcribed to cDNA using TAKARA. The TMPO-AS1 levels in tissues were determined by qRT-PCR analysis.
Results: qPCR assay confirmed that TMPO-AS1 was upregulated in gastric cancer tissues compared with normal tissues(p<0.017).
Conclusion: TMPO-AS1 was overexpressed in gastric tumor tissues compared with margin noncancerous tissues. Therefore, it can be concluded that TMPO-AS1 may be a potential diagnostic and prognostic biomarker to be used as a therapeutic target for GC; besides there is need for further researches to confirm these findings.