• Study of lncRNA TMPO-AS1 expression in gastric cancer
  • elaheh mohammadali,1 Reza Safaralizadeh,2,* behzad baradaran,3
    1. Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz., Tabriz., Iran
    2. Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz., Tabriz., Iran


  • Introduction: Gastric cancer is one of the most common cause of cancer-related death; besides, the diagnosis and therapy of it are still challenging. Researches have revealed that long non-coding RNAs(lncRNAs) play important roles in the genesis and progression of various human cancers including gastric cancer (GC). Investigations showed that TMPO-AS1 may be a diagnostic and prognostic marker in multiple cancer types. Thus, we selected TMPO-AS1 for further study in gastric cancer.
  • Methods: In the present study, solid tissue samples from 20 gastric tumors, and 20 normal tissues were obtained from gastric cancer patients. Afterward, the extracted RNAs were reverse-transcribed to cDNA using TAKARA. The TMPO-AS1 levels in tissues were determined by qRT-PCR analysis.
  • Results: qPCR assay confirmed that TMPO-AS1 was upregulated in gastric cancer tissues compared with normal tissues(p<0.017).
  • Conclusion: TMPO-AS1 was overexpressed in gastric tumor tissues compared with margin noncancerous tissues. Therefore, it can be concluded that TMPO-AS1 may be a potential diagnostic and prognostic biomarker to be used as a therapeutic target for GC; besides there is need for further researches to confirm these findings.
  • Keywords: Gastric cancer, lncRNA, Gene expression, TMPO-AS1, qRT-PCR