مقالات پذیرفته شده در سومین کنگره بین المللی زیست پزشکی
Differential Maturation of miR-17 ~ 92 Cluster Members in Human Cancer Cell Lines
Differential Maturation of miR-17 ~ 92 Cluster Members in Human Cancer Cell Lines
mozhgan abasi,1,*
1. Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran
Introduction: While some microRNAs are transcribed from a specific promoter, at least one third of human miRNA genes are clustered, wherein multiple miRNA genes are generated from a single primary transcript such as miR-17 ~ 92 cluster. Although six members of the cluster are generated from a single transcript, themature level of each member may be diverse in various cell types.
Methods: We attempt to monitor thematurelevelofmiR-17,miR-92a,andmiR-20afrommiR-17~92cluster in blood (HL60 (human promyelocytic leukemia cells) and Jurkat) and breast (MDA-MB-231 and
MCF-7) cancer cell lines. Interestingly, differentmature levels of themiRNAswere observed in each cell line. While miR-20 was highly matured in HL60 andMDA-MB-231 cell lines, higher mature
levelofmiR-92awasobservedinJurkat cell linecompared tothatofmiR-20andmiR-17.Further, the maturelevelofmiRNAswas alsomeasuredinnormalandcancercell lines
Results: Although the mature level of miR-17 and miR-92a increased in HL60 and Jurkat cell lines, miR-20 expression showed an almost identical level in blood cancer cell lines compared to controls. Conversely, miR-20 mature level significantly increased in breast cancer cell lineswhereas the expression level ofmiR-92a was comparable in MDA-MB-231, MCF-7, andMCF-10A cell lines
Conclusion: our results clearly demonstrated the diverse mature levels of individual miR-17 ~ 92 cluster members in different cell lines. While the molecular mechanisms responsible for differential
maturation of individual miRNA cluster members are unclear, it is tempting to speculate that post-transcriptional regulation of the miR cistron may have a key role in this process.