مقالات پذیرفته شده در سومین کنگره بین المللی زیست پزشکی
The effect of estrogen on β-tubulin (Tuj-1) gene expression in neural like cells differentiated from endometrial stem cells
The effect of estrogen on β-tubulin (Tuj-1) gene expression in neural like cells differentiated from endometrial stem cells
Elham Hasanzadeh,1Somayeh Ebrahimi-Barough,2Narges Mahmoodi,3Faezeh Esmaeili Ranjbar,4Houra Nekounam,5Jafar Ai,6,*
1. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran 2. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran 3. Sina Trauma and Surgery Research Center, Tehran University of Medical Sciences, Tehran, Iran 4. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran 5. Department of Medical Nanotechnology, School of Advanced Technology in Medicine, Tehran University of Medical Sciences, Tehran, Iran 6. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
Introduction: Improvement of stem cell differentiation to replace lost cells after spinal cord injury (SCI) has gained attention in recent years for spinal cord regeneration and repair. Estrogen is a female sex hormone which has neuromodulatory and neuroprotective effect on the central nervous system. In addition, estrogen has a cell plasticity effect and can induce the expression of nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) that are important for neuronal differentiation. Our study aimed to investigate the effect of estrogen on human endometrial stem cells (hEnSCs) differentiation into neuron-like cells in the presence of other signaling molecules and to investigate the expression of beta-tubulin III.
Methods: hEnSCs were isolated from endometrial biopsies using collagenase type I (1 mg/ml) and cultured in Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12) incubated at 37 °C in a humidified chamber (5% CO2). Isolated cells were characterized using flow cytometry for EnSCs markers. Passage 3 EnSCs were used for neural differentiation. Induction mediums composed of estrogen, retinoic acid (RA), fibroblast growth factor 2 (FGF2), epidermal growth factor (EGF), ITS and B27 Supplements. After 14 days of differentiation, the cells were evaluated by immunofluorescence (IF) and reverse transcriptase-real time polymerase chain reaction (RT-real time PCR) for beta-tubulin III (Tuj-1) marker.
Results: The results of RT-real time PCR and IF showed up-regulation of beta-tubulin III marker in gene and protein level respectively. Estrogen can improve neuron differentiation from hEnSC.
Conclusion: Estrogen is an important hormone with different effects on neural survival and physiology. The present study shows that estrogen can influence neural differentiation of EnSCs by increasing the expression of β-tubulin as a neural-specific marker.