مقالات پذیرفته شده در سومین کنگره بین المللی زیست پزشکی
Preparation of Silk Fibroin-based Nanofibers Containing Vitamin D3 for Bone Tissue Engineering Applications
Preparation of Silk Fibroin-based Nanofibers Containing Vitamin D3 for Bone Tissue Engineering Applications
parisa mostafavi,1mitra naeimi,2,*
1. Biomedical Engineering Department, Central Tehran Branch, Islamic Azad University, Tehran, Iran 2. Biomedical Engineering Department, Central Tehran Branch, Islamic Azad University, Tehran, Iran
Introduction: Bone defects have increased widely in recent years. Bone grafts are not adequate to meet clinical requirements, and limitations of autologous or heterologous grafts persist. Hence, bone tissue engineering is one of the most attractive research areas. Polymeric scaffolds may improve cell attachment and proliferation. The most important characteristics of silk fibroin (SF) scaffolds include tailorable biodegradability, cytocompatibility, and minimal inflammatory response. Polycaprolactone (PCL) is a linear hydrophobic polymer but its poor hydrophilicity causes a reduction in cell adhesion and proliferation. Electrospinning is an efficient technique to produce fibers with nano to micro diameter by altering polymeric solutions and process parameters. With the aim of developing a novel fibrous scaffold, in this study, silk fibroin (SF) and polycaprolactone (PCL) blended nanofibers were prepared via electrospinning method.
Methods: In order to prepare nanofibers, SF/PCL solutions were prepared in formic acid with ratios of 1/0 and 2/1 containing 0.1 % VD3. After blending the solutions were stirred at 200 rpm for 1 hour to obtain a homogenous solution. Electrospinning the solutions were performed at 12-25 kv and 0.1 ml/h. Scanning electron microscopy (SEM), contact angle measurement, mechanical properties, and MTT assay (MG63 cell culture) were used to characterize the scaffolds.
Results: According to SEM images, mean diameter of the nanofibers were in the range of 85±0.01 nm and 216±0.03 nm for 1/0 and 2/1 scaffolds, respectively. The mean diameter of the fibers was increased by adding SF and VD3 to PCL nanofibers. The results of contact angle measurements proved that blending SF and PCL could increase wettability of the surface. According to MTT assay, no cytotoxicity was observed and MG63 cell proliferation was improved for VD3 containing nanofibers. Cell cytotoxicity test showed that VD3 could increase cell viability and accelerate proliferation, after 48 hours in culture. Mechanical properties of SF/PCL fibers were also improved by incorporation of PCL.
Conclusion: In this study we produced SF/PCL/VD3 nanofibrous scaffolds with different ratios. PCL is a hydrophobic polymer. Wettability has major role in cell attachment and was changed by adding SF to PCL. The results showed that the properties of the scaffolds were strongly influenced by the addition of PCL and VD3 into SF nanofibers. The mean diameter of the fibers was increased and wettability of the surface was decreased in SF/PCL/VD3 scaffolds. Contact angle was reached to 53 degree for composite nanofibers. The results confirmed that SF/PCL/VD3 nanofibers may have potential use in bone regeneration.