• Study of IL-1 Genes Polymorphisms in HBs-Ag Positive and Normal Population of western azerbaijan province
  • mojtaba ranjbari,1,* Milad Esmatparast,2
    1. Department of biology, faculty of science, Islamic Azad University, Urumieh, Iran.
    2. Department of biology, faculty of science, Islamic Azad University, Urumieh, Iran.


  • Introduction: Genetic studies on multiple carriers have revealed that human leukocyte antigen (HLA (and various cytokine genes such as:)) 4-TNF-alpha, TNF-gamma, IL-12, IL-18, TGF-beta genes , IL-10, IL-1, IL (associated with HBV sensitivity or its durability or disease severity. Cytokines play an important role in defense against viral infection; IL-1 is also one of the cytokines. The most important members of the IL-1 family include IL-1alpha, IL-1beta, and IL-1RN, all of which are polymorphic, which is a major pro-inflammatory factor and plays a central role in inflammation and viral elimination. Polymorphisms are located in gene regulatory regions and have functional significance with They have a strong role in regulating IL-1 protein production and are associated with the spread of certain diseases. Studies in patients with hepatitis B in Japan have found that a link between IL-1beta polymorphisms and cirrhosis associated with hepatitis B Another study in the same country found that polymorphisms of this gene may be associated with predicting the risk of inflammatory diseases. Some studies on the polymorphisms of the ILRN1 gene also suggest that individuals with the 2-intron allele of the IL-1RN gene may be resistant to HBV infection.
  • Methods: Blood samples are stored in tubes containing anticoagulants and their DNA extracted using standard formula-ether. After determination of OD, the absorption coefficient at 260 and 280 nm wavelengths of DNA concentration per unit absorbance per ml / ml was obtained and the samples were finally diluted to 50 ng / ml and dissolved in Ependorf DNAase-free tubes. Store in the freezer below 20 ° C.
  • Results: In this project, the polymorphisms of interleukin 1 genes will be compared in two groups, the first group of patients with known HBs-Ag positive virus infection and the second group of completely healthy individuals without a history of anti-hepatitis B vaccine and They have no previous signs of HBs-Ab, HBc-Ab, hepatitis B.
  • Conclusion: Overall, we showed in this article that the frequency of alleles of HLA antigens and various cytokine genes such as IL-1, IL-2, IL-2, IL-2, TGF-beta, with or without HBV sensitivity, or The severity of the disease is related. There is a direct relationship between hepatitis B-associated IL۱-beta polymorphisms and cirrhosis. Whereas IL ۱ -RN polymorphisms are resistant to hepatitis B in alleles of the 2-intron IL-2RN gene.
  • Keywords: HBV - Polymorphism - HBs-Ag