مقالات پذیرفته شده در سومین کنگره بین المللی زیست پزشکی
Assessment of the effect of infliximab on immunomodulation properties of mesenchymal stem cells in vitro
Assessment of the effect of infliximab on immunomodulation properties of mesenchymal stem cells in vitro
yasaman aliasghari sakha,1abbas hajifathali,2,*ehsan ehsani,3elham roshandel,4arsalan jalili,5
1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran 2. Hematopoietic Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran 3. Department of Biology, Roudehen Branch, Islamic Azad University, Roudehen, Iran. 4. Hematopoietic Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran 5. Hematopoietic Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Introduction: Mesenchymal stem cells have immunosuppressive properties also differentiation properties in the treatment of inflammatory diseases. Tumor necrosis factor alpha (TNFα) and Interferon gamma (IFNγ) are major contributors for inflammatory conditions. In many inflammatory conditions, infliximab is used to treat patients that with blocking TNF lead to suppression of inflammation. The aim of this study is to investigate the effect of using infliximab on the anti-inflammatory performance of mesenchymal stem cells.
Methods: In this study, mesenchymal stem cells derived from bone marrow subsequently, with peripheral blood mononuclear cells from patients with inflammatory bowel disease who were in inflammatory conditions were cultured at doses of 10, 20, and 30 μg / ml for 48 and 72 h, respectively. The expression of IDO and iNOS genes, as well as the level of PGE-2 secretion in supernatant culture were investigated and the results were analyzed by SPSS and PRISM software.
Results: The results of this study showed that the expression of IDO and iNOS genes, as well as the level of PGE-2 secretion in solely cultivated Maroon mist, was the lowest and in the cultivation of MSCs with peripheral blood mononuclear cells are the most abundant.
Conclusion: Infliximab also has an effect on mesenchymal stem cells due to inhibition of TNF in inflammatory conditions and with reducing of the inflammatory signaling through this inflammatory factor leading to inhibition of the effective functioning of cells.