Introduction: Cancer is a worldwide disease which a small percentage of it, is inherited. Breast cancer is the most common heterogeneous disease which is diagnosed among a growing number of women around the world each year. Recent studies, have focused on the effect of microRNAs (miRNAs) on protein-coding genes such as oncogenes, tumor suppressor and anti-metastatic genes in breast cancer genetics. MicroRNAs (miRNAs) are a small class of noncoding RNAs that control gene expression by targeting mRNAs which inhibit translation or cause RNA degradation.
Methods: We figured validated target genes in miRTarBase and then predicted target genes in miRWalk. the expression of hsa-mir-342-3p target genes in normal and tumor tissues was found by GEPIA database and target genes with zero expressions were omitted. Also enrichment analysis via DAVID database was accomplished and impact of SNPs was accessed.
Results: Based on the results given, it has been found that hsa-mir-342-3p by effecting on p53, MAPK, ERbB, PI3K-AKT, wnt signaling pathways, adherens junction, focal adhesion and pathways in cancer had a certain role in breast cancer and also the correlation between important target genes such as cyclin, CDK, CASP, RTK, AKT, PAK was obtained by GeneMANIA database.
Conclusion: Hsa-mir-342-3p almost inhibits cell growth, cell proliferation, angiogenesis, metastasis and induces apoptosis and differentiation by affecting cyclin, CDK, AKT, PI3K, IKK genes in MAPK, ERbB, PI3K-AKT, Wnt singnaling pathways, adherens junction, Focal adhesion and pathways in breast cancer. We conclude that hsa-mir-342-3p is a tumor suppressor.
Keywords: Breast cancer, MicroRNA, Hsa-mir-342-3p, Enrichment analysis