مقالات پذیرفته شده در سومین کنگره بین المللی زیست پزشکی
Methylation of IL-6 promoter in subjects with type2 diabetes
Methylation of IL-6 promoter in subjects with type2 diabetes
Mehrnaz Sadat Ravari,1,*Mohammad javad sanaei,2masoud heshmati,3
1. Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran 2. Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran 3. Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
Introduction: Background and Aim: Type 2 diabetes (T2D) is a multifactorial illness and development of type 2 diabetes is associated with a combination of lifestyle and genetic factors. Epigenetic changes occur as a result of interference with environmental and lifestyle factors. Epigenetic can affect the function of the genes involved in the pathway of glucose metabolism. In the present study, the promoter methylation levels of candidate gene (IL-6) in patients with T2D and non-diabetic individuals were analyzed.
Methods: Material and methods: A total of our samples were 62 patients with T2D and 30 age- and gender-matched healthy controls. Lipid profiles were enzymatically measured and DNA extraction, bisulfite conversion, nested PCR and sequencing was performed to investigation of DNA methylation in IL-6 gene.
Results: Results: No differences were observed for the IL-6 gene promoter DNA methylation between type 2 diabetes and none diabetic subjects
Conclusion: Conclusion: Our findings suggest that the DNA methylation levels of the IL-6 promoter in blood are not related to the occurrence of T2D.
Keywords: Type2 Diabetes, DNA methylation, Interleukin-6,