مقالات پذیرفته شده در سومین کنگره بین المللی زیست پزشکی
Evaluation of pathogenicity of Crithidia parasites isolated from patients (Crithidia iranica) in BALB/c mice.
Evaluation of pathogenicity of Crithidia parasites isolated from patients (Crithidia iranica) in BALB/c mice.
Fatemeh Goudarzi,1,*Qasem Asgari,2Mohammad Hossein,3
1. Dept. of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran 2. Dept. of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran 3. Dept. of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Introduction: Leishmania major and Leishmania tropica have been considered the main agents of cutaneouse leishmaniasis in Iran. Furthermore, there are recent reports regarding to existence of trypanosamatidae family including Crithidia spp in cutaneous lesions, which drug resistance has been observed in several patients, and cutaneous ulcers are sometimes present in patients between two and three years and have been choronic. Therefore, we investigated the ability of the Crithidia iranica to infect BALB/c mice.
Methods: In order to investigate the morphology of the lesions caused by Leishmania major and Crithidia iranica, 40 female BALB/c mice and inbred were purchased from the Pasteur Institute and divided into 4 groups of 10. The first group was considered as positive control and 106 promastigotes of L. major in the stationary phase were inoculated in the base of their tail. The second group was infected with 106 promastigotes of C.iranica, the third group, after the injection of L. major and after emerging of lesions, the Critidia promastigotes were injected. In the last group, L. major and Crithidia were injected simultaneously. After inoculation of parasites, the mice were continuously followed and after the initial symptoms of ulcer in the base of their tail, the diameters of the lesions were measured every week for six weeks by the vernier caliper.
Results: The results of pathogencity of Crithidia in BALB/c mice showed that no lesion were observed in a group of mice receiving C. iranica and after statistical analysis, it was found that there was no significant difference in the area of the wound produced in the other three groups.
Conclusion: According to the results obtained, it can be deduced that Crithidia iranica can’t be an agant of cutaneous lesion lonely and the presence of this parasite with L. major does not affect the clinical course of the infection.