• The Investigation of in Vitro Toxicity Effect of Nickel Oxide Nanoparticles on Human Neuroblastoma Cell Line (SH-SY5Y)
  • Mozhdeh Hajimohammadjafartehrani,1 Mahsa Ale-Ebrahim,2,* Shahram Zarabiyan,3
    1. Department of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
    2. Department of Physiology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
    3. Department of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran


  • Introduction: Today, the nickel oxide nanoparticles have extremely been used in biotechnology and pharmaceutical fields. Although, nickel oxide nanoparticles can induce cell cytotoxicity in normal cells, the cytotoxicity of this nanoparticles against nervous system cells has not been explored yet.
  • Methods: In this in vitro study, the effect of different concentrations of nickel oxide nanoparticles (0, 1, 10, 20, 50, 100 μg / ml) on human neuroblastoma cells (SH-SY5Y) was determined by MTT, lactate dehydrogenase, caspase 9/3 activity evaluation. In this research, staining with acridine orange and ethidium bromide were analyzed. The results were analyzed with SPSS software and by one way ANOVA and student t test.
  • Results: : NiO NPs demonstrated a significant inhibition on the viability of SH-SY5Y cells and had a marginal impact on the LDH leakage. However, NiO NPs provided a significant effect on the elevation of activity of caspase-9/3 and the staining of acridine orange and ethidium bromide was also confirmed with these results.
  • Conclusion: This study determined that NiO NPs may mediate the induction of some undesired harmful effects on the neuron cells and this nanoparticles have toxic effects. This data may be attributed to the induction of apoptosis in SH-SY5Y cells by NiO NPs rather than necrosis induction.
  • Keywords: This study determined that NiO NPs may mediate the induction of some undesired harmful effects on th