مقالات پذیرفته شده در سومین کنگره بین المللی زیست پزشکی
Co-culture and co-transplantation of pancreatic islets with mesenchymal stem cells: hope for high insulin production and diabetes treatment
Co-culture and co-transplantation of pancreatic islets with mesenchymal stem cells: hope for high insulin production and diabetes treatment
Mona Navaei-Nigjeh,1,*
1. Pharmaceutical Sciences Research Center, The Institute of Pharmaceutical Sciences (TIPS), Tehran University of Medical Sciences, Tehran, Iran.
Introduction: Islet transplantation is a curable option for the patients with insulin-dependent diabetes mellitus. Owing to the inherent lack of islet donors, exploring the replacement of islets gives rise to the boom of stem cell research in this field. Mesenchymal stem cells (MSCs) in adult tissues, such as bone marrow and adipose tissues, have the capacity of self-renewal and potential to differentiate into different cell types.
Methods: In recent years, these cells have attracted significant attention due to their potent immunomodulatory properties activated by the surrounding microenvironment, where abundant inflammatory factors are released from immune cells. In addition, MSCs have been shown to secrete several paracrine molecules, which mediate trophic effects on neighboring cells, enhancing viability and function of islets during in vitro culture and in vivo transplantation. Moreover, MSCs are known to help speed up the process of vascularization and angiogenesis, which can attenuate the hypoxia and ischemia conditions after islet transplantation.
Results: As evidenced by co-transplantation experiments with the best glycemic control and glucose response, the direct interaction between MSCs and pancreatic islets allowed the building of a symbiotic microenvironment and led to the increased viability and function of islets in vivo. Thus, MSCs are promising feeder or supportive cells in islet transplantation therapy.
Conclusion: In conclusion, it was demonstrated that co-culture of pancreatic islets with MSCs has the potential for protecting islets from injury after isolation and during culture period and co-transplantation of islets with MSCs has a beneficial effect on the outcome of islet grafts for treatment of diabetic hyperglycaemia.