مقالات پذیرفته شده در سومین کنگره بین المللی زیست پزشکی
Expression profile of pro-inflammatory cytokines in SPF chickens after administration of inactivated Newcastle antigen and Flagellin
Expression profile of pro-inflammatory cytokines in SPF chickens after administration of inactivated Newcastle antigen and Flagellin
Maryam Barkhordari,1Masoumeh Bagheri,2,*Saeed Irian,3
1. Kharazmi University, Faculty of Biological Sciences, Department of Cell and Molecular Biology, Tehran, Iran. 2. Razi Vaccine and Serum Research Institute (RVSRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran. 3. Kharazmi University, Faculty of Biological Sciences, Department of Cell and Molecular Biology, Tehran, Iran.
Introduction: Since none of the Newcastle vaccine formulations show the perfect protection, effective vaccines are necessary to be established. Flagellin (FliC) as an adjuvant for vaccine improvement can activate the TLR5 on the surface of immune cells and help the immune system in better recognition of antigens. TLR5 is a strong stimulator of proinflammatory cytokines such as IL-6 and TNF- α.
Methods: In this research investigation the efficacy of flagellin along with the inactivated Newcastle virus antigen was evaluated in a complete random design using a total of 56 SPF chickens in 6 groups (PBS as control, antigen (Ag), Ag+full length FliC (flFliC), Ag+Native FliC (NFliC) Ag+truncated FliC (trFliC) and inactivated commercial vaccine). Immunization was performed twice in 10 and 24 days post-hatching. Eighteen hours following the second immunization, bloods were drawn and total RNA was purified. Immediately using oligodT primers, cDNA was synthesized and real-time quantitative PCR was performed using specific primers. Immunogenicity of all treatment groups were evaluated in SPF chickens, and the expression levels of tumor necrotic factor-α (TNF-α) and interleukin-6 (IL-6) were relatively analyzed by Real-Time-PCR.
Results: The results revealed an up-regulation of the expression of IL-6 and TNF- α genes after 18 hours post immunization in the treatment groups Ag+full length FliC, Ag+Native FliC and Ag+truncated FliC (p<0.05), with flFliC group having the greates change.
Conclusion: It is concluded that using flagellin as a Newcastle disease vaccine adjuvant can induce proinflammatory cytokines, and thus presents a suitable adjuvant.