مقالات پذیرفته شده در سومین کنگره بین المللی زیست پزشکی
Prepare innate, natural condition to enhance proliferation of mesenchymal stem cell
Prepare innate, natural condition to enhance proliferation of mesenchymal stem cell
Maria Zahiri,1,*Banafshe Esmaeilzade,2Ahmad Ashourinejad,3
1. 1. Department of Anatomical Sciences, School of Medical Sciences, Bushehr University of Medical Sciences, Bushehr, Iran 2. 1. Department of Anatomical Sciences, School of Medical Sciences, Bushehr University of Medical Sciences, Bushehr, Iran. 3. School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
Introduction: The behavior of Mesenchymal stem cells (MSCs) depends on the paracrine and biophysical signals received from the 3D micro-environment. These cells are of great interest due to their high potency to differentiate The simultaneous use of suitable nanofibrous scaffolds and conditional media can simulate the microenvironment needed by stem cells. The aim of this study is to fabricate and evaluate the effect of Tissue-engineered scaffold of Poly (L-lactic acid) (PLLA) and conditional medium on mesenchymal stem cell culture.
Methods: By electrospinning method, Poly (L-lactic acid) (PLLA) nanofibrous porous scaffolds were prepared. Characterization of scaffolds was investigated using scanning electron microscopy. Bone marrow derived MSCs was confirmed by osteogenic and chondrogenic differentiation and flow cytometric analysis and then cultured in the PLLA scaffold in the presence of MSCs conditional medium. Cellular biocompatibility of nanoscafold was assessed using by SEM. The viability and proliferation of the cells was then determined by an MTT assay.
Results: Histological and flow cytometric analysis confirmed bone marrow derived MSCs. SEM data confirmed the porosity of scaffold with suitable pore size. The MTT assays revealed the biocompatibility of PLLA nano scaffold under conditional medium.
Conclusion: The 3D architecture nanofibrous scaffold and conditional stem cell can improve the viability and proliferation MSCs.