مقالات پذیرفته شده در سومین کنگره بین المللی زیست پزشکی
Down-regulation of miR-429 and 214-3p inhibit ovary/uterus cancer cell migration, proliferation and metastasis by blocking ACT, RAS, and PAK2
Down-regulation of miR-429 and 214-3p inhibit ovary/uterus cancer cell migration, proliferation and metastasis by blocking ACT, RAS, and PAK2
setare samizade,1,*alireza nasr esfahani,2negar balmeh,3masoumeh masah,4
1. islamic azad univercity najafabad 2. islamic azad univercity najafabad 3. Zistfanavary Novin ,Biotechnology Institute , Esfahan, Iran 4. Department of Biochemistry, Islamic Azad University of Najafabad . Department of Biotechnology. Nonprofit University of Ashrafi Esfahani.Researcher Biotechnology Institute, Esfahan, Iran
Introduction: MicroRNAs (miRNA), are endogenous 19-25 nucleotide noncoding single stranded RNAs that regulates gene expression by blocking the translation or decreasing the stability of mRNAs. Dysregulation of microRNA expression plays a major role in the development and progression of most human malignancies and cancer. Down regulation of mir-429 and mir-214-3p is associated with ovary and uterus cancer. So this study aims to obtain the role of oncogenic genes Act, Ras, PAK2 by mir-429, 214-3p in ovary and uterus cancer.
Methods: MicroRNA specifications was accrued by using mirbase. Then the valid and predict genes were identified from mirtarbase and mirwalk2.0. Finally, pathways archived from KEGG and David. GENEMANIA was used to find gene network.
Results: The result indicated that mir-429 and 214-3p by inhibiting Ras which actives Rac and TIAM1, block PAK2, so cell motility and cytoskeleton organization changes will be block and cancer suppresses. Mentioned microRNAs inhibit JUN by blocking NCK and PAK2 which active JNK and JNKK by phosphorylation. So JUN through the inhibition of angiogenesis, prevent cancer development. In other side of the pathway mir-429 and 214-3p effect on adhesion increasing by preventing cell migration and inhibit proliferation by blocking Ras which actives Raf, MEK, ERK , MYK through phosphorylation and suppress cancer.
Conclusion: mir-429, 214-3p effect on cell motility, cytoskeleton organization, angiogenesis, proliferation and adhesion of cancer cell. Mentioned microRNAs by effecting on Act, PAK2, Ras in ErB, MAPK, RAS pathways inhibit proliferation, angiogenesis, metastasis by effecting on adhesion increasing, preventing cell migration and cytoskeleton organization changes. Finally mir-429 and 214-3p, prevent the development and spread of the tumor as tumor suppressor.