• Investigating the association between mir-429, 214-3p, 6726-3p and breast cancer in Erb/MAPK pathways
  • setare samizade,1,* alireza nasr esfahani,2 masoumeh masah,3 negar balmeh,4
    1. islamic azad univercity najafabad
    2. islamic azad univercity najafabad
    3. Department of Biochemistry, Islamic Azad University of Najafabad . Department of Biotechnology. Nonprofit University of Ashrafi Esfahani.Researcher Biotechnology Institute, Esfahan, Iran
    4. Zistfanavary Novin ,Biotechnology Institute , Esfahan, Iran


  • Introduction: Breast cancer (BRCA), is the most commonly diagnosed cancer in women, and the second leading cause of cancer death in the world. Erb/MAPK pathways play crucial role in regulation of cancer cell and its malignancy. MicroRNAs (miRNA), are small noncoding RNAs that are emerging as important modulators in cellular pathways and appear to play a role in tumorigenesis, cell cycle, proliferation and apoptosis. So this study purposes to evaluate the association between hsa-mir-429, 214-3p, 6726-3p and breast cancer in ERB/MAPK pathways.
  • Methods: Specifications of miRNAs were obtained through mirbase. The mirtarbase and MIRWALK2.0 target genes were identified. Using NCBI, all target genes were expressed in normal and breast cancer tissue and the signaling pathways of the common target genes were observed from the DAVID database and the pathways associated with breast cancer were stored for interpretation. The network of genes was obtained from GENE MANIA.
  • Results: The result demonstrated that mir-429, 214-3p, 6726-3p inhibit JUN by blocking NCK and PAK2 which actives JNK and JNKK by phosphorylation. So JUN through inhibition angiogenesis, suppresses cancer. In other side of the pathway mentioned microRNAs effect on adhesion increasing by preventing cell migration and inhibit proliferation by blocking Ras which actives Raf, MEK, ERK, MYK through phosphorylation and suppresses cancer.
  • Conclusion: mir-429, 214-3p and 6726-3p effect on angiogenesis, proliferation and cell adhesion. Mentioned microRNAs by effecting on Act, PAK2, JUN, Ras in ERB/MAPK pathways, act as tumor suppressor and inhibit angiogenesis, proliferation and effect on adhesion increasing by preventing cell migration. Finally mir-429, 214-3p,-6726-3p inhibit cancer cell growth and suppress cancer.
  • Keywords: MicroRNA, Erb/MAPK pathways, Breast cancer