مقالات پذیرفته شده در سومین کنگره بین المللی زیست پزشکی
MicroRNAs-199a-5p, 3120-3p, 215-5p,194-5p,664a-3p prevent development of cancer cell and tumor spread by directly targeting oncogenic genes in lung cancer
MicroRNAs-199a-5p, 3120-3p, 215-5p,194-5p,664a-3p prevent development of cancer cell and tumor spread by directly targeting oncogenic genes in lung cancer
Alireza Nasr Esfahani,1,*Setareh Samizadeh,2Negar Balme,3Masoumeh Masah,4
1. Islamic Azad University Najafabad Esfahan 2. Islamic Azad University Najafabad Esfahan 3. Zistfanavary Novin Biotechnology Institute Esfahan 4. M.Sc. Department of Biochemistry, Islamic Azad University of Najafabad .Department of Biotechnology. Nonprofit University of Ashrafi Esfahani.Researcher Biotechnology Institute,Esfahan,Iran
Introduction: Lung cancer is the number one cancer killer in the world from both men and women. Lung cancer is a disease in which certain cells in the lung became abnormal and multiply uncontrollably to form a tumor and may spread to lymph nodes or other organs in body. MicroRNAs, are small noncoding RNAs which regulate gene expression primarily at the posttranscriptional level and their expression profile improve classification diagnosis and prognostic information of malignancies including lung cancer as oncogene or tumor suppressor. Here in, this study investigates the communication between mir-199a-5p, 3120-3p, 215-5p,194-5p,664a-3p and lung cancer in cellular pathways.
Methods: By using mirbase, miRNA properties were obtained. The mirtarbase and MIRWALK2.0 target genes were identified. Through NCBI, the expression of target genes in the normal and lung cancer tissue was obtained. Using DAVID, signal paths were obtained and the pathways associated with lung cancer were interpreted. The gene network was obtained through GENE MANIA.
Results: The result demonstrated that mir-199a-5p, 3120-3p, 215-5p,194-5p and 664a-3p inhibit ERK by blocking Ras, SOS which active Raf, MEK through phosphorylation. So ERK by blocking ELK, MYC effect on adhesion increasing through preventing cell migration and suppress cancer. Mentioned microRNA by inhibiting EGFR, Ras, SOS, ERK inhibit proliferation of cancer cell and tumor spread. MicroRNAs , by inhibiting Ras, PI3K take their stimulation impact of PIP3 and block Act, IKK, NFkB and prevent cell migration and metastasis.
Conclusion: Mir-199a-5p, 3120-3p, 215-5p,194-5p and 664a-3p effect on metastasis, proliferation and adhesion of cancer cell. Mentioned microRNAs by effecting on Act, Ras, SOS, EGFR, MAPK1, PI3K in ERB, Non-small cell lung cancer, RAS, PI3-ACT pathways, inhibit proliferation and metastasis by effecting on adhesion increasing and prevent cell migration. Therefore MicroRNAs act as tumor suppressor and destroy cancer by preventing the development of cancer cell and spread of tumor.