مقالات پذیرفته شده در سومین کنگره بین المللی زیست پزشکی
Mesenchymal stem cells encapsulated in gel for Retinal tissue engineering
Mesenchymal stem cells encapsulated in gel for Retinal tissue engineering
Mostafa Soleimannejad,1,*Somayeh Ebrahimi-Barough,2Ramak Roohipoor,3Masoud Soleimani,4Jafar Ai,5Mohammad Riazi-Esfahani,6
1. Department of Tissue Engineering, School of Advanced Technologies in Medicine, Shahrekord University of Medical Sciences, Shahrekord , Iran 2. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran 3. Eye Research Center, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran 4. Department of Hematology and Blood Banking, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran 5. Department of Tissue Engineering, School of Advanced Technologies in Medicine, Shahrekord University of Medical Sciences, Shahrekord , Iran 6. Gavin Herbert Eye Institute, University of California, Irvine, CA, USA
Introduction: Retinal detachment dysfunction has been shown as a key phenomenon in the AMD diseases and RP. The application of stem cells with hydrogel can be extensively advantageous based on growing proof of these stem cells.
Stem cell-based therapies are attraction approaches for tissue engineering and regenerative medicine for remedying retinal diseases. Fibrin gel, a bio-polymeric material with excellent biocompatibility, provides numerous advantages as a tissue engineering scaffold and a stem cell carrier.
Methods: After isolation of stem cells CJMSCs, the cells were differentiated into photoreceptor-like cells by exposing with taurin for 21 days in tissue culture plate (TCP group) and fibrin hydrogel (3 D group). The attachment of cells was analyzed with SEM and MTT. The expression of rhodopsin, PKC, recoverin, peripherin, nestin, CRX, and RPE65 as photoreceptor-like cell markers was evaluated by immunocytochemistry and quantitative real-time PCR (RT-PCR) in TCP and 3 D groups. The results of SEM analysis showed CJMSCs were well attached in fibrin gels and there was good integrity between cells and scaffold.
Results: After 3 weeks, the appearance of rhodopsin, PKC, recoverin, nestin, CRX, peripherin and RPE65 as special markers of photoreceptor cells were detected by Real-time PCR and immunofluorescence that these presentation in 3 D groups were higher than TCP groups.
Conclusion: Our findings showed that presentation of readily available sources of adult stem cells like human conjunctiva stem cells encapsulated in fibrin gel could be a stimulating plan to enhance photoreceptor progenitor cell numbers for repair and regeneration of retinal disease such as photoreceptor damage.