مقالات پذیرفته شده در سومین کنگره بین المللی زیست پزشکی
A comparative study of three methods for determining the drug susceptibility pattern of Mycobacterium tuberculosis
A comparative study of three methods for determining the drug susceptibility pattern of Mycobacterium tuberculosis
Ehsan Aryan,1,*Gholamali Azari Garmjan,2Masoud Yousefi,3Hadi Safdari,4
1. Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences 2. Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences 3. Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences 4. Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences
Introduction: Timely determination of drug susceptibility pattern (DSP) of Mycobacterium tuberculosis (Mtb) plays an important role in choosing the appropriate treatment regimen for tuberculosis (TB) patients. Therefore, a method for determining DSP of Mtb, especially for using in developing countries, should be easy, inexpensive, reliable and efficient. The aim of this study was to compare the efficacy of two relatively new rapid, easy and inexpensive methods, microscopic observation drug susceptibility (MODS) and nitrate reductase assay (NRA), with the conventional and standard drug susceptibility method (proportions) in identifying the resistance of Mtb to the four first-line drugs in the treatment of TB.
Methods: In a cross-sectional study, 52 Mtb isolates were collected and evaluated between October 2013 and June 2014 from Regional TB Reference Laboratory, Shariati hospital, Mashhad, Northeast Iran.
Results: In this study, NRA and MODS showed very good agreement for detection of resistance to isoniazid and rifampin (κ 0.9, p <0.001). However, both methods showed poor agreement (κ 0.5) for detection of resistance to ethambutol. Moreover, the turnaround time for NRA and MODS was 15.3 and 9 days, respectively, compared to 38 days needed for the standard proportions method.
Conclusion: Considering the accuracy of NRA and MODS results as well as the turnaround time of both methods compared to the standard proportions method, MODS seems to be a more appropriate approach in determination of DSP of Mtb, especially in low-income countries.