• preparation of new nanoparticle-based vaccine against prostate cancer
  • Negin Zia,1,* Nowruz Delirezh,2 Sanaz Sheikhzadeh,3
    1. Department of Microbiology , Faculty of Veterinary Medicine , Urmia University , Urmia IRAN
    2. Department of Microbiology , Faculty of Veterinary Medicine , Urmia University , Urmia IRAN
    3. Department of Microbiology , Faculty of Veterinary Medicine , Urmia University , Urmia IRAN


  • Introduction: Preparation of new nanoparticle-based vaccine against prostate cancer Negin zia, Nowruz delirezh, Sanaz Sheikhzadeh Prostate cancer (PC) is the second common cause of cancer-related death, cancer vaccines have the least toxic effects and are designed to stimulate immune cells to target specific TAAs that are overexpressed in cancer cells. The production of nanoparticle-based cancer vaccines and the delivery of cancer antigens along with immunostimulatory adjuvants to dendritic cells in vivo have led to an excellent development in cancer vaccine efficacy, Poly (D, L-lactide-co-glycolic acid (PLGA) is a biodegradable polymer that approved by the FDA as a human drug delivery system, The purpose of this study was to produce a novel delivery approach for tumor antigens and adjuvants in order to enhance efficiency of antigen delivery for anti-tumor vaccines.
  • Methods: The LNCAP tumor cell lysate was obtained from Four cycles of freeze-thaw and the protein concentration of the lysate was measured by a Bradford protein assay. Then tumor cell lysate (as an antigen) and Poly (I:C) (as an adjuvant) encapsulated in PLGA copolymers (50% lactide: 50% glycolide) using the solvent evaporation method from a water/oil/water (W2/O/W1) emulsion. The nanoparticles were lyophilized and then average particle size, polydispersity index and Zeta potential of nanoparticles were assessed by performing a dynamic light scattering (DLS).
  • Results: Lyophilized particles were dispersed in distilled water and submitted to analysis of size, polydispersity index, and Zeta potential. The average diameter was 224 nm with PdI=0.4. The average zeta potential was −4.5 mV. Physicochemical properties of nanoparticles, such as size, polydispersity index, and surface charge, play a key role in the cellular uptake of nanoparticles with dendritic cells. The successful production of nanoparticles with desirable properties was one of the results of this study that can be used as a prostate cancer vaccine to stimulate dendritic cells.
  • Conclusion: The present study suggested that the use of nanoparticles in vaccine formulations allows not only improved antigen stability and immunogenicity but also targeted delivery and slow release.
  • Keywords: PLGA nanoparticles, Tumor cell lysate, Poly (I:C), Cancer vaccine