• The effects of Zinc Oxide nanoparticles on histological structure of liver tissue in Black Molly Poecilia sphenops
  • Ziba Kolouei,1 Tahereh Naji,2,* Homayoun Hosseinzadeh,3 Negin Rabiei,4
    1. Department of Basic Sciences, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
    2. Department of Basic Sciences, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
    3. Iranian Fisheries Science Research Institute, Agricultural Research and Education Organization, Tehran, Iran.
    4. Department of Basic Sciences, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.


  • Introduction: zinc oxide nanoparticles due to their unique properties and diverse applications including antibacterial antifungal and antiviral properties and its use in weather purification and preservatives have been considered These nanoparticles because of its small size it is well absorbed by the nanoparticles and accumulates in the tissues over time causing toxicity Metal oxide nanoparticles such as zinc oxide have many applications in cosmetics
  • Methods: in this study the effect of the Zinc Oxide nanoparticles on histological structure of liver tissue were examined. For this purpose 90 pieces of fish with an average weight of 4.2 gr consist of 4 treatment groups doses 2-4-6-10 ppm of Zinc Oxide nanoparticles and 2 group of control that received 20 micro liter of distilled water were prepared Fish had injection every other day for 20 days then liver tissue were dissected and histology of liver were examined
  • Results: the results of this study indicate that with increasing dose of zinc oxide nanoparticles HSI% decreased there was a significant difference between the HSI% of the control group without injection and the group receiving 10 ppm zinc oxide nanoparticle the observation of liver tissue sections showed the adverse effect of zinc oxide nanoparticles on liver tissue in control groups hepatocytes were normal and were observed at normal levels sinusoids were healthy and no bleeding was seen the groups receiving the highest dose of zinc oxide nanoparticle showed apoptosis and deformity of hepatocytes bleeding between hepatocytes was also observed hepatocytes were small and sinosoids were wide
  • Conclusion: evaluation of hepatosomatc index data shows that with increasing dose of these nanoparticles in both male and females we have decreased liver weight and decreased HSI% this decrease also occurs due to necrosis and apoptosis of the liver cells liver tissue of treatments zinc oxide nanoparticles in doses of 6 and 10 ppm were observed hepatocyte deformation shrinkage hemorrhage and dilation of sinusoids
  • Keywords: zinc oxide nanoparticles, Liver, Poecilia sphenops, Hepatocyte