مقالات پذیرفته شده در سومین کنگره بین المللی زیست پزشکی
Modified graphene oxide for neural tissue engineering
Modified graphene oxide for neural tissue engineering
Ayda Yari Ilkhchi,1abbas ebrahimi kalan,2Mehrdad Mahkam,3,*
1. Department of chemistry, faculty of science, Azerbaijan Shahid Madani University, Tabriz, Iran 2. Tabriz University of Medical Sciences, Neurosciences Department, Tabriz, Iran 3. Department of chemistry, faculty of science, Azerbaijan Shahid Madani University, Tabriz, Iran
Introduction: Central nervous system (CNS) lesions, incorporate of traumatic brain injury, spinal cord injury and neurological diseases such as Alzheimer, Parkinson, and Huntington, because of the limited regenerative ability of nerve tissue, is difficult to restore. In the CNS system injuries, particularly in spinal cord injury (SCI), glial scar tissue produced by astrocytes is the main factor that prevents nerve regeneration. Additionally, the meningeal cell functionality and the lack of Schwann cells reduce axonal growth, nowadays near two million people travail of spinal cord injury globally.
Graphene based materials have been widely exploited in different biomedical research areas such as bioimaging, drug delivery, gene delivery, bioelectronics, biosensors and tissue engineering due to their superb electrical and thermal conductivity, biocompatibility, exclusive surface structure and geometry.
Methods: In this study, incorporation possibility of the tremendous physicochemical properties of GO with biomaterials for nerve regeneration was inquired. Briefly, after synthesis of graphene oxide through the modified Hummer method, functionalized by polyethylene glycol and chitosan. Follow up the chemical and morphological analysis; in vitro tests on mesenchymal stem cells and in vivo on mice model were investigated.
Results: Synthesis and modification of graphene-based materials were characterized using Fourier transform infrared spectroscopy (FT-IR), X-ray Diffraction (XRD), and scanning electron microscopy (SEM) techniques and cytotoxic effect were studied by MTT assay. Modified Go was implanted in mice spinal cord injured (T10 level of spinal cord) and investigated its influences on nerve tissue and recovery potential.
Conclusion: We have implanted flexible modified GO scaffolds at the T10 level of injured mice spinal cord and explored the injured nerve tissue reaction and mice movement restoration. According to the obtained results, these flexible compounds could be facilitated tissue regeneration one week after spinal cord injury and prevented the development of additional scar fabrication and lack of systemic and local toxic effects.