• No association between GPX1 promoter methylation and type 2 diabetes
  • Mehrnaz Sadat Ravari,1,* Effat Farrokhi,2 zaha moradi shahgharieh,3 mohammad javad sanaei,4
    1. Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
    2. Department of Molecular Medicine, School of Advanced Technologies, Shahrekord University of Medical Sciences, Shahrekord, Iran
    3. Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran


  • Introduction: Background and Aim: Type 2 Diabetes (T2D) is a multifactorial and slowly progressing multiple organ disease, and development of type 2 diabetes is associated with a combination of lifestyle and genetic factors. Epigenetic changes occur as a result of interference with environmental and lifestyle factors. Epigenetic can affect the function of the genes involved in the pathway of glucose metabolism. One of the Epigenetic factors are DNA methylation. DNA methylation has been shown to function as cellular memory, and it provides the major mechanism by which expression patterns and response to stimuli can become heritable over many cell generations. The aim of this study is to investigate promoter methylation levels of candidate gene (GPX1) in patients with T2D and non-diabetic individuals.
  • Methods: Methods: A total of our samples were 62 patients with T2D and 30 age- and gender-matched healthy controls. Lipid profiles were enzymatically measured and DNA extraction, bisulfite conversion, nested PCR and sequencing was performed to investigation of DNA methylation in GPX1 gene.
  • Results: Results: No differences were observed for the GPX1 gene promoter DNA methylation between type 2 diabetes and none diabetic subjects. But our results revealed that methylation of GPX1 gene promoter had significantly higher methylation in females compared with males (p<0.05
  • Conclusion: Conclusion: Our findings suggest that the DNA methylation levels of the GPX1 promoter in blood are not related to the occurrence of T2D.
  • Keywords: Type2 Diabetes, DNA methylation, Glutathione peroxidase 1,