مقالات پذیرفته شده در سومین کنگره بین المللی زیست پزشکی
PCR detection of Listeria monocytogenes in women with idiopathic abortion
PCR detection of Listeria monocytogenes in women with idiopathic abortion
Haniyeh Nazari,1Mojtaba Sadeh,2,*Reza Hosseini Doost,3
2. Department of Microbiology, Faculty of Advance Science and Technology, Tehran Medical Science, Islamic Azad University, Tehran, Iran 3. Department of Microbiology, Faculty of Advance Science and Technology, Tehran Medical Science, Islamic Azad University, Tehran, Iran
Introduction: According to the World Health Organization reports, 15% of women worldwide have miscarriage and infertility, and 70% of them occur in the first trimester. Infectious agents are among the contributing factors to infertility and miscarriage. Several infectious factors cause miscarriage and infertility. Listeria monocytogenes are among the infectious pathogens in women and its early diagnostic can be a useful in treatment and prevention. The purpose of this study was to identify Listeria monocytogenes in women with idiopathic abortion with PCR.
Methods: In this study, 50 blood plasma samples from women with idiopathic miscarriage were collected. DNA was extracted from patients' samples by boiling / DNG-plus. The PCR test with Target Gene 16SrRNA was optimized using standard strain DNA and Limit of detection (LOD) and specificity were assessed. Finally, PCR test was performed on 50 samples, along with positive and negative controls.
Results: The PCR test was optimized and Amplicon 226bp was observed in Agarose gel. In Specificity test , only DNA Listeria monocytogenes was positive. Two of the samples (4%) were positive LOD of optimized test was 100 copy/reaction.
Conclusion: One of the most important causes of idiopathic abortions can be infectious agents such as Listeria monocytogenes, which are not commonly diagnosed in many laboratories. This study demonstrates that approximately 4% of idiopathic miscarriages can be associated with Listeria monocytogenes. In addition, the PCR test is a precise, sensitive and rapid test to diagnose this bacterium.