مقالات پذیرفته شده در سومین کنگره بین المللی زیست پزشکی
Folliculogenesis Genes Expression in Human Vitrified Ovarian Tissue After Xenotransplantation
Folliculogenesis Genes Expression in Human Vitrified Ovarian Tissue After Xenotransplantation
Zahra Shams Mofarahe,1 Marefat Ghaffari Novin,2Mojdeh Salehnia,3,*
1. Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran 2. Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran 3. Dept. of Anatomical Sciences, Tarbiat Modares University, Tehran, Iran
Introduction: Autograft transplantation of vitrified cortical ovarian tissue is an acceptable clinical technique for fertility preservation in women. Xenograft transplantation into animal models could be useful for evaluating the safety of human vitrified ovarian tissue. This study targeted to evaluate the impact of vitrification on the expression of genes associated with folliculogenesis after the xenograft transplantation of human vitrified ovarian tissue to γ-irradiated mice.
Methods: In this study, ovarian biopsies were gathered from six transsexual persons. The cortical section of ovarian biopsies was separated and chopped into small pieces. These pieces were randomly divided into vitrified and non-vitrified groups. In each group some pieces were considered as non-transplanted tissues and the others were transplanted to γ-irradiated female National Medical Research Institute (NMRI) mice. Before and after two weeks of xenograft transplantation, histological assessment and evaluation of the expression of folliculogenesis- associated genes (FIGLA, GDF-9, KL and FSHR) were performed in both vitrified and non-vitrified groups.
Results: The percentages of the normal follicles and the expression of all examined genes of transplanted and non-transplanted tissue were resemble in both vitrified and non-vitrified groups (P>0.05). After transplantation, the normal follicle rate significantly decreased and among the folliculogenesis associated genes, the expression of GDF-9 gene was significantly increased, compared with before transplantation in vitrified and non-vitrified tissues (P<0.05).
Conclusion: The vitrification method using DMSO, EG, had no remarkable effect on the normal follicular rate and expression of folliculogenesis associated genes after 2 weeks human ovarian tissue xenografting. Also the transplantation process can cause a significant decrease in normal follicular rate and expression of GDF-9 gene.