مقالات پذیرفته شده در سومین کنگره بین المللی زیست پزشکی
Expression of mir-129, mir-146, mir-183, and mir-197 in Follicular Thyroid Carcinoma and Adenoma tissues
Expression of mir-129, mir-146, mir-183, and mir-197 in Follicular Thyroid Carcinoma and Adenoma tissues
sara sheikholeslami,1marjan zarif yeganeh,2,*setareh shivaee,3mehdi hedayati,4seyed mohammad tavangar,5
1. cellular and molecular endocrine research center, research institute for endocrine sciences, shahid beheshti university of medical sciences 2. cellular and molecular endocrine research center, research institute for endocrine sciences, shahid beheshti university of medical sciences 3. cellular and molecular endocrine research center, research institute for endocrine sciences, shahid beheshti university of medical sciences 4. cellular and molecular endocrine research center, research institute for endocrine sciences, shahid beheshti university of medical sciences
Introduction: Follicular thyroid carcinoma (FTC) is responsible for about 10% of thyroid malignancies. Since this type of malignancy does not show capsular and vascular invasions, adenoma and follicular carcinoma of thyroid are not distinguishable. It has been proved that microRNAs, which regulate approximately 30% of coding proteins, have an association with follicular thyroid adenoma (FTA) and carcinoma of the thyroid. Therefore, the aim of this study was to assess the expression of some miRNAs to detect the most appropriate miRNA as potential biomarkers in the diagnosis of FTA and FTC patients
Methods: Reverse transcription quantitative polymerase chain reaction (RT-qPCR) was applied to determine the expression levels of miR-129, miR-146,-183 and miR-197 in 16 FTC, 16 FTA and 16 hyperplasia/ multinodular goiter (MNG) cases. The significance of miRNA differential expression levels between groups were assessed using Multivariate test by Statistical Package for Science Software (SPSS v.20) and Graph Pad Prism v.8.
Results: Results showed that all of the miRNAs had significant overexpression in FTC and FTA versus MNG cases and also, increased expression in FTC compared to FTA but it was not significant. The results of ROC curve analysis determined the significant overexpression and prognostic value of miR-129 in FTA cases and miR-146 in both FTA and FTC cases compared to MNG group.
Conclusion: Although all of the mentioned microRNA overexpressed in FTC and FTA cases but the ROC curve results demonstrated that miR-129 had agreeable AUC for FTA cases, so it seems that it`s cut-off point could be helpful to distinguishing between FTA and multinodular goiter cases. On the other hand, although miR-146 has excellent diagnostic value in both FTA and FTC groups, but it seems this microRNA is unable to be as a specific biomarker to discriminate between FTA and FTC cases. This data need to confirm in large cohort study and other biological samples such as plasma.