مقالات پذیرفته شده در سومین کنگره بین المللی زیست پزشکی
Cytotoxicity Evaluation of Root, Leaf, Stem and flower of Dandelion (Taraxacum Peregrinum) on MCF7 Cancerous Cell Lines
Cytotoxicity Evaluation of Root, Leaf, Stem and flower of Dandelion (Taraxacum Peregrinum) on MCF7 Cancerous Cell Lines
Hoda Abolhasani,1,*Fatemeh heidari,2Ahmad Abolhasani,3
1. Cellular and Molecular Research Center & Departement of Pharmacology, School of Medicine, Qom University of Medical Sciences, Qom, Iran. 2. Department of Anatomy, School of Medicine, Qom University of Medical Sciences, Qom, Iran. 3. Department of Biotechnology, Faculty of Advanced Sciences and Technologies, Isfahan University, Isfahan, Iran
Introduction: Cancer is the second cause of death after cardiovascular diseases. With due attention to rapid progress in the phytochemical study of plants, they are becoming popular because of their anticancer effects. A study reported that among 65 new drugs registered for cancer treatment during 1981-2002, 48 drugs were obtained from natural products including: vinca alkaloids (vinblastine, vincristine, vindesine, vinorelbine), taxanes (paclitaxel, docetaxel), podophyllotoxin and its derivations (topothecan, irinothecan) and Anthra cyclines (doxorubicin, daunorubicin, epirubicin, idarubicin). One of the herbs that have been studied extensively in recent years for its anti-cancer and anti-inflammatory activity is dandelion (Taraxacum sp) extracts. The aim of this study is to investigate the cytotoxicity effects of hydroalcoholic extracts of Taraxacum Peregrinum on MCF7 cancerous cell line.
Methods: In this study, cytotoxic activity of hydroalcoholic extracts of root, leaf, stem and flower of Dandelion (Taraxacum Peregrinum) were evaluated as anticancer agents and were compared with cisplatin as a well-known anti-cancer drug. The cytotoxic effects of hydroalcoholic extracts of dandelion and cisplatin on MCF7 (breast) cell line were evaluated by MTT assay at 48 hours at seven concentrations (10000, 1000, 100, 10, 1, 0.1, 0.01 µM). ELISA reader was used at 570 nm wavelengths to determine the survival of cells. IC50 values were calculated by fitting the data in a sigmoidal dose-response curve by non-linear regression analysis using graphpad PRISM software (version 6.01) for each cell line.
Results: Based on the results of biological evaluations of this study, hydroalcoholic extracts of dandelion's root and stem have growth inhibitory effects comparable to cisplatin on MCF7 cancerous cell line.
Conclusion: The results of this study propose that hydroalcoholic extracts of dandelion's root and stem are suitable for investigating of new herbal anti-cancer drugs.