• Evaluation the presence of Staphylococcus aureus and cytomegalovirus (CMV) genomes in serum samples of type 2 diabetes patients
  • Negin Azadan,1,* Fariba khosravi ,2 Atosa Ferdousi,3 Mohammad Hassan shahhosseini,4
    1. Department of Microbiology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran
    2. Department of Microbiology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran
    3. Department of Microbiology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran
    4. Iranian Gene Fanavar Institute (IGF), Tehran, Iran


  • Introduction: Some recent investigations point to the role of microorganisms as a possible causative factor for diabetes type 2. Among these agents, Staphylococcus aureus and cytomegalovirus (CMV) as a bacterial and viral agent have been proposed by different studies respectively. Due to the novelty of this idea, still, there is not enough literature in this regard and needs to be approved by more experiments. Our aim was to assess this hypothesis by detecting their genomes in serum samples of diabetic patients.
  • Methods: 50 serum samples belong to the diabetic patients as the test group, and 50 serum samples belong to the non-diabetic persons as the control group were selected randomly. To finding the probable relationship between control of blood sugar and presence the genome of microorganisms in blood serum, the A1C parameter for diabetic patients was recorded too. In total these 100 serums were tested by PCR optimized tests for detecting genomes of Staphylococcus aureus and also cytomegalovirus. In the case of the mentioned bacterium, the specific primers NUC1 and NUC2 and Nuclease gene as the target gene with 279 bp product were considered. To detect CMV genome the Glycoprotein B with 257 bp as the target gene and specific primers CMV257F and CMV257R were applied. Both PCR tests were optimized and their sensitivity and specificity were evaluated too. Then the result between test and control group were compared.
  • Results: After running the PCR tests, none of the 100 samples, were positive for Staphylococcus aureus and cytomegalovirus. In terms of staphylococcus aureus, the related amplicon band was just observed for the positive control of the test and the results relate to the CMV were the same. Obviously no relationship between A1C parameter and microorganisms genomes presence was observed.
  • Conclusion: Based on our obtained results, can be concluded the proposed idea about the role of microorganisms to trigger diabetic type 2 cannot be true in all of the cases or the possibility of that is not too high. The result also shows that in the case of bacterial or viral infections there was no difference between the diabetic group and the control group. In addition, the results show that the level of blood sugar didn’t have any effect in this regard. Our results didn’t approve the role of these microorganisms to develop diabetes, to be more precise should be said the incidence is not very high and still needs to be tested by more samples.
  • Keywords: PCR, CMV, type 2 diabetes, Staphylococcus aureus