Introduction: Recurrent spontaneous abortion (RSA) is defined as the occurrence of two or more consecutive pregnancy losses prior to 20th week of gestation. HLA-G is a non-classical molecule of type Ib MHC which is mainly expressed on the fetal trophoblastic cells and has been suggested to play some critical roles in maintaining the fetal–maternal relationship. Here we investigated the association of rs1632943 from upstream of the HLA-G gene with the recurrent spontaneous abortion in the Northwest women of Iran.
Methods: In this case-control study, 100 women with history of RSA as a case group and 80 healthy women with one or more than one children as the control group were included. Genomic DNA was purified from their peripheral blood samples and their genotypes were identified using PCR-sequencing method. Fisher's exact test was used for comparing the genotype and allele frequencies between the case and control groups.
Results: In the rs1632943 polymorphism the frequency of CC, CA and AA genotypes were 8%, 33% and 59% in the patient group and 16.25%, 43.75% and % 40 in the control group, respectively. Frequency of C and A alleles in patients was 24.5% and 75.5% and in control group was 38.125 % and 61.875%, respectively. Statistical analysis showed that AA genotype was associated with the recurrent spontaneous abortion (P = 0.005, OR=2.159). Moreover, the allele A was identified as a risk allele for recurrent spontaneous abortion (P = 0.014, OR=1.899).
Conclusion: Results of present study highlighted that the rs1632943 polymorphism might be considered as a risk factor for RSA in the northwestern Iranian women.