• Evaluation the effect of chitosan nanoparticles on bap gene expression involved in biofilm formation of MDR ACINETOBACTER BUMANNII isolates usig Real time PCR.
  • Tohid Piri Gharaghie,1,* Seyed Ataollah Sadat Shandiz2,2 hossein amin anaraki,3
    1. shahrkord azad university
    2. central tehran branch university
    3. tehran shargh university


  • Introduction: Acinetobacter borne is one of the most common opportunistic pathogen in hospital that is resistant to many antibiotics due to the production of biofilms. The aim of this study was to evaluation of anti-biofilm activity of chitosan nanoparticles on clinical antibiotic resistant Acientobacter bumanni.
  • Methods: In this experimental study, chitosan nanoparticles was purchase from Pishgaman nanomavad Iranian and subsequently, Acientobacter bumanni isolates were isolates from 100 clinical samples. After identification of Acientobacter bummani strains and determination of antibiotic resistant profiles, biofilm producer isolates were determined using PCR method. After determination of chitosan nanoparticles MIC and treatment of strains with subMIC concentration of chitosan nanoparticle, RNA extraction and cDNA synthesis was performed. Finally, gene expression of biofilm gene (Bap) was measured using Real Time PCR.
  • Results: Among 100 clinical isolates, 12 isolates was belonged to Acientobacter bummani and all of strains were resistant to antibiotics except colistin. PCR results show that 12 isolates have Bap gene and they were biofilm positive. Real Time PCR results show that after treatment of isolates with subMIC concentration of chitosan nanoparticles, all of strains had a significant reduction in bap gene expression (P<0.05).
  • Conclusion: According to anti-biofilm effects of silver nanoparticles, it is appear that chitosan nanoparticles can be used as drug candidate in pharmaceutical industries.
  • Keywords: Acientobacter bummani, chitosan nanoparticle, Biofilm, Real Time PCR