Introduction: Hospital infections are infections that the patient did not suffer at the time of admission and was not in the commune period. In recent years, nosocomial infections have had a huge outbreak and are a serious problem for health centers, and they are imposing high costs on health centers and hospitals. One of the most important causes of acute infection is the Acinetobacter, which is recognized as a dangerous pathogen in the intensive care unit. The aim of this study was to investigate the synergistic effects of silver nanoparticles with alcoholic extract of Puneh and Nettle extract against resistant multi-drug Acinetobacter isolates
Methods: 300 bacterial samples were collected from ICU sections of different hospitals in Tehran. 250 Acinetobacter isolates were identified by diagnostic tests. Antibiotic test was then performed by disc diffusion method for Acinetobacter spp. And resistant isolates were isolated. Antibacterial effects of Puneh and Nettle extract and Nanoparticles were evaluated individually, as well as in combination with non-growth halo methods, MIC and MBC tests, and differential-inhibitory concentration(FIC).
Results: The results showed that 0.65 μg / ml of silver nanoparticle had antitumor antibacterial properties for all Actinobacteria. Puneh and nettle extracts had antibacterial properties against Acinetobacteria resistant isolates at concentrations )1.40 and 2.81 μg / ml( and standard Acinetobacteria strains at concentrations )1.81 and 3.62 μg / ml(. Among the dicotyledons, according to the amount of FIC, the combination of nettle extract with silver nanoparticles against the standard isolate and resistant isolate had a synergistic effect, while the combination of Puneia extract with silver nanoparticles against standard isolates and resistant isolates had an incremental effect.
Conclusion: Achieving antibacterial compounds of plant origin is very important given the antibiotic resistance of the last decade. With the correct use of antibiotics and the low cost of combining extracts with silver nanoparticles , these compounds can be used in conjunction with antibiotics in the treatment of solutions or even in the form of plant fibers.