مقالات پذیرفته شده در سومین کنگره بین المللی زیست پزشکی
Prostaglanding F-2 alfa stimulate the secreation of VEGF factor in migration of adipose tissue derived mesenchymal stem cells
Prostaglanding F-2 alfa stimulate the secreation of VEGF factor in migration of adipose tissue derived mesenchymal stem cells
samira shomali,1,*Abdolkhaleg Deezagi,2
1. Payame Noor university 2. Department of Molecular Medicine and Biochemistry, National Institute of Genetic Engineering and Biotechnology
Introduction: Today , Tissue engineering uses factors that can induce differentiation of mesenchymal stem cells (MSCs) into other cell types.However, the problem of angiogenesis in this differentiated tissue remains an unresolved area of research interest. The aim of this study was to investigate the effects of prostaglandind F-2alfa (PEG-2alfa) on the expression of vascular endothelial growth factore (VEGF) in human adipose tissue derived MSCs.
Methods: In this experimental research, human adipose tissue was digested using collagenase. The isolated MSCs cells were treated with PGF-2alfa (up to 5 microg/ml) and incubated for 96 hours.
Cell migration were spontaneously assayed by scratching methods.
Results: Cell growth at 1.0 , 2.5 , 5 micro g/ml of PGF-2alfa was not significantly reduced compared to control cells ,suggesting that these concentrations of PGF-2alfa are not toxic to cell growth.The scratching test also demonstrated a positive influence on cell proliferation and migration.
Cells treated with 1.0 microg/ml of PGF-2 alfa for 12 hours showed the highest relative migration and coverage in comparison to untreated cells.
Conclusion: Stimulation of VEGF secretion by PGF-2alfa treated MSCs could useful for the induction of angiogenesis in tissue engineering in vitro.